Type
SandwichELISA,Biotin-labelledantibody
Applications
Serum
SampleRequirements
mousesamples:7 µl/well
ratsamples:50 µl/well
Storage/Expiration
Storethecompletekitat2–8°C.Undertheseconditions,thekitisstableuntiltheexpirationdate(seelabelonthe box).
CalibrationCurve
CalibrationRange
40–2560pg/ml
LimitofDetection
18.4 pg/ml
Intra-assay(Within-Run)
mousesamplesCV=8.4%
ratsamplesCV=8.5%
Inter-assay(Run-to-Run)
mousesamplesCV=8.7%
ratsamplesCV=6%
SpikingRecovery
mousesamples98.9%
ratsamples104.5
DilutationLinearity
mousesamples99.8%
ratsamples103.7%
Crossreactivity
- bovineWeakreactivity(recom.dilution1:3)
- catYes(recommendeddilution1:3)
- dogNon-detectable
- horseNon-detectable
- goatYes(recommendeddilution1:6)
- hamsterYes(recommendeddilution1:12)
- humanNon-detectable
- monkeyNon-detectable
- rabbitNon-detectable
- chickenNottested
- mouseYes
- ratYes
- sheepYes(recommendeddilution1:3)
- pigYes(recommendeddilution1:3)
